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LSM-60F is a new confocal laser scanning microscope, which can realize high-precision observation and accurate analysis. It can be widely used in morphology, physiology, immunology, genetics, and other fields, and is an ideal partner for cutting-edge biomedical research.
It scans samples sequentially point by point, or multiple points at once. The pixel information is assembled into an image. As a result you acquire optical sections with high contrast and high resolution in x, y and z.
● High signal-to-noise ratio: High-efficiency confocal imaging optical path, even under weak fluorescence can also provide extremely high signal-to-noise ratio fluorescence images
● Excellent image: wide spectrum, high numerical aperture lens, perfect for all types of confocal sample shooting
● Easy to use: fully electric frame, optimized design of human-machine interaction interface, so that you can be comfortable in the process of sample shooting
LSM-60F Biological Applications
All-electric control system
● The Z-axis adopts a motorized device, which can quickly adjust the Z-axis height according to the real-time image. AF one-button autofocus, eliminating fine-tuning steps and improving work efficiency.
● The integrated control buttons on both sides of the fuselage can realize the quick switching or rotation of the condenser, brightness, objective lens, attenuator turntable, and fluorescence turntable, improving the convenience of operation.
Superior confocal imaging optics
● Unique imaging pinhole structure: Minimize interference caused by component displacement, improving image signal-to-noise ratio and axial resolution
● Controller detection unit: high-sensitivity detection unit (maximum QE≥45%@500nm), which can easily and quickly complete multi-color fluorescence confocal imaging automatically
Objective lens
● LSM-60F laser scanning confocal microscope has two sets of objective lenses, apochromatic (2X-100X) and super-apochromatic (10X-100X), with a wide range of magnification, suitable for advanced research microscopy and microscopic image shooting. Large numerical aperture to further improve resolution.
Confocal dedicated software
LSM-60F confocal microscopes also have powerful functional software. it supports single-channel or multi-channel two-dimensional imaging (XY), three-dimensional imaging (XYZ), four-dimensional imaging (XYZT), and multi-site scanning. Imaging, photobleaching, and photostimulation can be performed within a user-defined ROI (region of interest), Z-stack imaging, large image stitching, ruler correction, filtering, data logging, and more.
Specification:
1) Laser light source
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Laser
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405nm/50mW、 488nm/50mW、 561nm/50mW、 640nm/40mW
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Laser control
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Direct modulation and acoustooptic modulation
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2) Scanning module
Scanning unit
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Biaxial XY high-speed optical scanning galvanometer
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Field of view 14mm×14mm(≥ 19)
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Scan pixels 512×512-4096×4096
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Pixel time 0.5μs-8μs
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Standard scanning speed: 0.9fps(512×512， 2μs); Fast scan speed: 3fps(512×512, 0.5μs)
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Scanning unit
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Biaxial XY high-speed optical scanning galvanometer
Field of view 14mm×14mm(≥ 19)
Scan pixels 512×512-4096×4096
Pixel time 0.5μs-8μs
Standard scanning speed: 0.9fps(512×512， 2μs); Fast scan speed: 3fps(512×512, 0.5μs)
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Pinhole
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The circular pinhole, located between the dichroic spectroscope and the scanning galvanometer, ensures that the fluorescence acquisition efficiency of the system is always 100%
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Dichroic spectroscope
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Four-channel dichroic spectrometer： 405/488/561/640nm
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Filter
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The 6-hole electric filter wheel is standard with four pieces: 445nm/40, 530nm/43, 607nm/36 and 685/40
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Confocal detection
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MA PMT, QE ≥ 25%@500nm, 20%@600nm (PMT quantity 1)
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Unit
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GaAsP PMT, QE ≥ 45%@500nm, 40%@600nm
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DIC Detection unit
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With differential interference imaging function, can realize "DIC- fluorescence" imaging
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3) Research grade inverted microscope
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Optical system
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Infinite chromatic aberration correction optical system
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Viewing head
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Tilting trinocular viewing head, 20-45° degree adjustable, inter-pupillary range: 50-76mm
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Eyepieces
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High eye-point wide field plan eyepiece PL10X/22mm, with adjustable diopter, micrometer attachable
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Objective
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Infinity Plan semi-apochromatic objectives 4x, 10x. 20x. 40x. 60x. 100x
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Frame
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The low-hand coarse and micro coaxial electric focusing mechanism, the front large-size LCD touch display panel, and the body integrated electric control buttons, can realize the rapid switching of condenser, brightness, objective lens, optical port, and other functions. Built-in electric glazing port, located on the left side of the fuselage, splitting ratio 100:0, 0:100; built-in electric left optical port, splitting ratio 0:100, 50:50, 100:0; optional single-layer / double-layer optical path, provides system expansion space, and can switch each layer of optical path modules with one click as needed. Z-axis travel 10.5mm
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Working stage
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Manual mechanical platform, table size 300mm(X)×240mm(Y), moving range 135mm(X)×85mm(Y)
Electric stage, the size of the table is not less than 260mm(X)×153mm(Y), and the moving range is 110mm(X)×75mm(Y), with an independent operating handle; The maximum speed is 3mm/S, and the repeatability of positioning is ±1um
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Condenser
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Motorized 7-hole condenser, NA ≥ 0.55, WD ≥ 27mm; 3 holes for φ30mm (phase contrast), 4 holes for φ38mm (DIC); support brightfield, phase contrast, DIC observation (including polarizer group)
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Fluorescent lighting system
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8-hole fluorescent turntable system, the system can determine the position of the turntable placed on the upper and lower layers of the rack; and has an electric shutter function, which can directly block the fluorescent lighting light source, with fluorescent filter block lens group: B1/G1/UV1, etc.
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4) Operating software
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Scanning imaging
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Image acquisition and system automatic control function, all-electric control switching of the optical path; adaptive camera parameters and preview parameters; full field of view and ROI scanning imaging; monochromatic or multi-color two-dimensional imaging (XY), three-dimensional imaging (XYZ), four-dimensional imaging (XYZT) and multi-site scanning
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Processing analysis
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Multicolor fluorescence co-localization processing, fluorescence, and differential interference (DIC) image overlay; correction and addition of rulers; filtering processing; Z-Stack processing analysis and large image stitching
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